Warunya
Panmanee
, Ph.D.
Postdoctoral Fellow
2000-2003: Ph.D. (Biotechnology) , Mahidol University , Bangkok , Thailand
1995-1999: M.Sc. (Biochemistry; The first of class) , Mahidol University , Bangkok , Thailand
1992-1995: B.Sc. (Biotechnology) , Prince of Songkla University , Songkla , Thailand
AWARDS AND SCHOLARSHIPS
2000-2003: Royal Golden Jubilee Scholarship
1999: Graduate studies of Mahidol University Alumni Association
1996-97: National Science and Technology Development AgencyScholarship for
Graduate Student
1992-95: Prince of Songkla University Undergraduate Student
Scholarship
WORKING EXPERIENCES
January 2005- present: Postdoctoral Fellow at Department of Molecular
Genetics, Biochemistry and Microbiology
Hassett Lab, University of Cincinnati, College of Medicine
Cincinnati, Ohio, USA
August 2003-2004: Postdoctoral Fellow at Laboratory of Biotechnology,
Chulabhorn Research Institute, Bangkok, Thailand
August 1999-April 2000: Assistant Reseacher at Laboratory of Biotechnology,
Chulabhorn Research Institute Institute, Bangkok,
Thailand
1997-1998: Teaching Assistance at Department of Biochemistry,
Mahidol University, Thailand
RESEARCH TRAINING
Laboratory training at Department of Biochenmistry Wake Forest University School of Medicine, Medical Center Boulevard, North Calorina, USA with Assoc. Prof. Leslie B. Poole
INVITED PRESENTATIONS
The study of new family negative transcription regulator, organic hydroperoxide resistance regulator (ohrR), in Xanthomonas campetris pv. phaseoli at The 27th Congress on Science and Technology of Thailand, Songkhla Thailand, October 2001
Studying of OhrR, anovel trancriptioal regulator, in sensing and responding to organic hydroperoxide at RGJ-Congress IV, Pattaya Thailand , April 2003
OhrR: Mechanism of organic hydroperoxide sensing in gram negative bacteria at Microbial Stress Response at Annual TRF Senior Research Scholar Mini-conference, Chulabhorn Research Institute, Bangkok, Thailand , January 2003
ABSTRACT PRESENTATIONS
Alterations of E6 gene expression in etoposide resistance in cervical cancer at 25th Congress on Science and Technology of Thailand, Pitsanuloke Thailand , October 1999
Unusual adaptive and cross-protective responses against peroxide killing in Vibrio harveyi at The 12th Annual Meeting of the Thai Society for Biotechnology, Kanchanaburi, Thailand, November 2000
The study of OhrR, a novel transcription repressor that responds to changes in organic peroxide levels at Microbial Stress Response Gordon Research Conference, Rhoad Island USA, July 2002
OhrR, The sensor and regulator in responding to organic hydroperoxide at The fifth Princess Chulabhorn International Science Congress, Bangkok Thailand, August 2004
OhrR, sensing of organic hydroperoxides at The 8th Asian Conference on Transcription (ACT-VIII), Chulabhorn Research Institute, Bangkok Thailand, November 2004
Role of Psedomonas aeruginosa OxyR in biofilm resistance to hydrogenpeoxide at The American society for Microbiogy ASM 105th General Meeting, Atlata Georgia, USA, June 2005
OprL, a peptidoglycan associated lipoprotein, is required for protection of an oxyR mutant from hydrogen peroxide mediated killing under aerobic conditions at The American society for Microbiogy ASM 107th General Meeting, Toronto Ontario, Canada, May 2007
Anaerobic survival of Pseudomonas aeruginosa mutants devoid of the quorum sensing regulator RhlR and the anti-sigma factor MucA is dependent on medium pH and terminal electron acceptor at The American society for Microbiogy ASM 107th General Meeting, Toronto Ontario, Canada , May 2007
A Pseudmonas aeruginosa oxyR mutant requires catalse (KatB) for protection against H2O2 in planktonic culture while it requires alkyl hydroperoxidase (AhpCF) in biofilms. at The American society for Microbiogy ASM 108th General Meeting, Boston, USA, June 2008
SKILLS
Recombinant DNA Techniques, Cloning, Southern blot analysis, Western blot analysis, Northern blot analysis, Site-directed mutagenesis, Primer extention, Enzymatic assays such as -galactosidase activity, DNA mobility shift assay, Protein expression and Purification, Mammalian cell culture, PCR, FPLC and HPLC, 2D gel electrophoresis, MALDI-TOF MS
Selected Publications:
- (2008) The peptidoglycan- associated lipoprotein OprL helps protect a Pseudomonas aeruginosa mutant devoid of the transactivator OxyR from hydrogen peroxide-mediated killing during planktonic and biofilm culture. J. Bacteriol. 90(10):3658-69
- (2007) Structural mechanism of organic hydroperoxide induction of the transcription regulator OhrR. Mol Cell.30;28(4):652-64.
- (2006) 2006 Novel organic hydroperoxide-sensing and responding mechanisms for OhrR, a major Bacterial Sensor and regulator of organic hydroperoxide stress. J Bacteriol. 188(4):13899-95.
- (2005) Novel roles of ohrR-ohr in Xanthomonas sensing, metabolism, and physiological adaptive respond to lipid hydroperoxide. J Bacteriol. 187(9):3277-81
- (2003) Induction of peroxide and superoxide protective enzymes and physiogical cross protection against peroxide killing by a superoxide generator in Vibrio harveyi. FEMS Microbiol Lett, FEMS Microbiol.Lett. 221: 89-95.
- (2002) OhrR, a transcription repressor that senses and responds to changes in organic peroxide levels in Xanthomonas campestris pv. phaseoli. Mol Micro 45 (6): 1647-1654.
- (2002) The repressor for an organic peroxide-inducible operon is uniquely regulatedd at multiple levels. Mol Micro. 44 (3): 793-802.
- (2002) Evalution of the roles that alkyl hydroperoxide reductase and Ohr play in organic peroxide-induced gene expression and protection against organic peroxides in Xanthomonas campestris Biochem Biophy Res Com. 299: 177-182
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